Cannot create SQL connection after Server Security Hardening

[kamiller]

Help our ASP.NET Application Fails to create SQL Connection after
Server Security Hardening

System worked perfectly until Security ran "Hardening Scripts"

Errors caught by ExceptionManagement Application Block

Error Description: System.UnauthorizedAccessException

Message: Access is denied.

Error Message Source is System.EnterpriseServices

Stack Trace follows: at
System.EnterpriseServices.Platform.Initialize() at
System.EnterpriseServices.ResourcePool..ctor(TransactionEndDelegate
cb) at System.Data.SqlClient.ConnectionPool..ctor(DefaultPoolControl
ctrl) at
System.Data.SqlClient.PoolManager.FindOrCreatePool(DefaultPoolControl
ctrl) at
System.Data.SqlClient.SqlConnectionPoolManager.GetPooledConnection(SqlConnectionString
options, Boolean& isInTransaction) at
System.Data.SqlClient.SqlConnection.Open() at
Microsoft.ApplicationBlocks.Data.SqlHelper.FillDataset(String
connectionString, CommandType commandType, String commandText,
DataSet dataSet, String[] tableNames, SqlParameter[]
commandParameters) at RO.GroupInfo.GetUserSecurityGroups(String
InAuthUser, String& SecurityGroupsXml)