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Message: [QUOTE="Dr.Ruud, post: 4802797"] Rita schreef: Whitespace must be expensive at your end. Please insert white lines before and after and inside code, and add spaces to make your code better readable, like: my $result = $in->next_result; my $hit = $result->next_hit; my $hsp1 = $hit->next_hsp; my $start1 = $hsp1->query->start; my $end1 = $hsp1->query->end; #-Print Starting and Ending Point of 1st Hsps- print "Start 1st Hsp ", $start1, "\n"; print "End 1st Hsp ", $end1, "\n\n"; my $hsp2 = $hit->next_hsp; my $start2 = $hsp2->query->start; my $end2 = $hsp2->query->end; #-Print Starting and Ending Point of 2nd Hsps- print "Start 2nd Hsp ", $start2, "\n"; print "End 2nd Hsp ", $end2, "\n\n"; print "Gap Sequence- ", $seq1->subseq($end1 + 1, $start2 - 1); Capitals and interpunction are also a good investment. It is my experience that people who make a mess when writing a natural language, tend to do similar when writing other stuff. [/QUOTE]