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Message: [QUOTE="Sachin, post: 492971"] Scenario: Machine A: ASP.NET Web UI IIS 6.0 Windows Server 2003 Impersonation Account: domain\Acct1 Machine B: ASP.NET Web Service IIS 6.0 Windsow Server 2003 Impersonation Account: domain\Acct2 when Web UI calls Web Service, it fails saying "System.Web.Exception The request failed with HTTP status 401: Unauthorized." When I look at the IIS log for Web Service I see, WebService.asmx -> HTTP Code, 401 2 WebService.asmx -> HTTP Code, 401 3 WebService.asmx -> HTTP Code, 401 1 domain\Acct1 has full control over web service web site and all the files underneath it. I used filemon on WebService.asmx and found that IIS is even not tring to read it. Then I deployed the same Web Service on Machine C, with similar configuration. Web UI works fine. Could someone please help me to find out what could be wrong? Any pointer? Thanks in advace Sachin [/QUOTE]