The server is not operational

[eduardo.ernandes]

I am with an intermittent problem in my Asp.Net application.
Occurs the error "The server is not operational" in the authentication
of the LDAP after the application to go off an error of Timeout in
some attempt of access to the database " Timeout expired. Prior The
timeout period elapsed you completion of the operation or the server
is not responding."
Somebody knows some solution for this problem?

ASP.NET 2.0
Enterprise Library 3.0 April 2007
Sql Server 2000
Windows Server 2003 R2
Enterprise x64 Edition
Service Pack 1
15.9 GB of RAM